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Updated: Oct 1, 2026
Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
Studies on isolation, serum-free cultivation and manufacture of mink enteritis virus optimized for vaccine
1Chinese Army Laboratory of Molecular Virology & Genetic Engineering, Institute of Preventive Medicine & Veterinary Science in Beijing Military Area of Chinese PLA, Fengtai District, P.R. China.
Abstract:
Optimum conditions have been determined for growth of mink enteritis virus (MEV) in the feline kidney cell (FKC) lines. The S18 and S36 strains of MEV grow to titres of 8192 to 32,786 haemagglutination (HA) units or 10(-9.7-10.3) TCID50 by alternate cultivation for 22 in FKC with very different susceptibility. Mink with typical clinical symptoms of mink viral enteritis (MVE) eliminated intestinal mucosal cylinders from which the L12 strain of MEV was isolated, which is capable of replication to 8192 HA units or 10(9.7) TCID50. The S18, L12 and S36 strains of MEV grow to high titres and the inactivated virus vaccine induced a good immune response in mink. The S18 and L12 strains of MEV are the best strains for vaccine preparation and the L12 strain is the most virulent for challenge tests.
Insights
Optimum growth conditions were identified for mink enteritis virus (MEV) in feline kidney cell (FKC) lines. Strains S18 and L12 are recommended for mink viral enteritis (MVE) vaccine development due to high viral titers and immunogenicity.
Area of Science:
- Veterinary Virology
- Cell Culture Technology
Background:
- Mink viral enteritis (MVE) is a significant disease affecting mink populations.
- Effective vaccine development requires optimizing virus propagation and identifying virulent strains.
Purpose of the Study:
- To determine optimal conditions for growing mink enteritis virus (MEV) in feline kidney cell (FKC) lines.
- To evaluate MEV strains for vaccine preparation and challenge testing.
Main Methods:
- Cultivation of MEV strains (S18, S36, L12) in FKC lines under various conditions.
- Measurement of viral growth using hemagglutination (HA) units and 50% tissue culture infectious dose (TCID50).
- Isolation of MEV from intestinal mucosal cylinders of infected mink.
Main Results:
- MEV strains S18 and S36 achieved high titers (8192-32,786 HA units or 10(-9.7-10.3) TCID50) in FKC lines.
- MEV strain L12, isolated from infected mink, replicated to high titers (8192 HA units or 10(9.7) TCID50).
- An inactivated virus vaccine using these strains induced a robust immune response in mink.
Conclusions:
- Strains S18 and L12 of MEV are suitable for inactivated vaccine production.
- Strain L12 is identified as the most virulent strain for challenge tests in vaccine efficacy studies.
