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Fluid shear stress increases the release of platelet derived growth factor BB (PDGF BB) by aortic endothelial cells
C Aromatario1, A V Sterpetti, R Palumbo
1I Istituto di Clinica Chirurgica, University of Rome, La Sapienza.
Objectives:
The aim of this study was to determine the correlation between shear stress and the release of Platelet Derived Growth Factor (PDGF BB) by aortic endothelial cells.
Design And Setting:
Laboratory in vitro study.
Materials:
Bovine aortic endothelial cells were seeded in fibronectin-coated cylinders at 1.0 x 10(6) cells/tube and allowed to reach confluence and to adhere for 48 hours. The experimental groups were subjected to nonpulsatile, laminar flow of 50, 100, 150 ml/min in polystyrene cylinders (i.d. 10 mm) of a closed circulatory loop giving a shear stress on the endothelial cells of 3, 6, 9 dyn/cm2. The control group was subjected to similar incubation conditions without flow.
Outcome Measures:
The release of PDGF BB by endothelial cells was measured by ELISA and Western Blot Analysis.
Results:
Shear stress increased significantly (p < 0.01) the release of PDGF BB by endothelial cells.
Conclusions:
PDGF BB release by endothelial cells may be one of the mechanisms linking hemodynamic forces and adaptation of blood vessels wall.