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Updated: Aug 17, 2026
Impression Cytology of the Lid Wiper Area
Published on: August 9, 2016
DNA ploidy and proliferating cell nuclear antigen image analysis of peritoneal and pleural effusions. A possible
A H el-Habashi1, S M Freeman, B el-Morsi
1Pathology Department, Cairo University, Egypt.
Objective:
To determine the role of DNA and proliferating cell nuclear antigen (PCNA) image analysis (IA) in enhancing the diagnostic sensitivity of conventional cytology (CC).
Study Design:
The histopathologic and clinical data on 87 consecutive pleural and peritoneal effusions were used to evaluate the accuracy of CC and DNA IA results.
Results:
CC showed a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 65%, 100%, 100% and 62%, respectively. Aneuploidy peaks were seen in 49 cases; 47 of them were true positives. Thirty of 38 diploid cases were true negatives. The sensitivity, specificity, PPV and NPV were 85%, 94%, 96% and 80%, respectively. There were positive correlations between DNA ploidy profile and PCNA proliferative index (PI), (R = .697) and significant differences in PCNA PI between malignant and benign effusions (P < .001).
Conclusion:
The DNA IA PI by PCNA can be used as a complementary diagnostic tool with CC in cytologically inconclusive cases.
