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Perfluorochemicals and cell biotechnology

K C Lowe1, P Anthony, J Wardrop

  • 1Department of Life Science, University of Nottingham, University Park, U.K.

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
|May 1, 1997
PubMed
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Perfluorochemical (PFC) liquids enhance cell culture by improving gas exchange for microbial, animal, and plant cells. These applications in biotechnology offer significant potential for increased biomass and product yields.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Biochemistry

Background:

  • Perfluorochemicals (PFCs) possess high gas solubility, a property valuable in biomedical and biotechnological applications.
  • PFCs are utilized to enhance respiratory gas supply for various cell types, potentially boosting biomass production and yields of valuable cellular products.

Purpose of the Study:

  • To provide a comprehensive overview of the applications and benefits of PFCs in microbial, animal, and plant cell culture systems.
  • To compare PFCs with alternative methods for managing respiratory gas supply in cell cultures.

Main Methods:

  • Review of existing literature on PFC applications in cell culture.
  • Comparative analysis of PFCs against other physical and chemical methods for gas supply manipulation.

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Main Results:

  • PFC liquids facilitate improved respiratory gas supply to prokaryotic and eukaryotic cells.
  • Culturing cells at the interface of PFC liquids and aqueous media has been demonstrated for animal and plant cells.
  • Fluorocarbon polymers serve as effective gas-permeable membranes in eukaryotic cell cultures.

Conclusions:

  • PFCs offer significant advantages in cell culture technology by optimizing gas exchange.
  • The use of PFCs can lead to enhanced biomass production and higher yields of commercially important cellular products.
  • PFC-facilitated cell culture improvements hold considerable biotechnological implications for the future.