Liposomes containing alpha-tocopherol and ascorbate are protected from an external oxidant stress
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Updated: Nov 1, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
Published on: March 3, 2020
R E Waters1, L L White, J M May
1Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Abstract:
The interaction between alpha-tocopherol and ascorbate in protecting membrane lipids from peroxidation was studied in unilamellar liposomes in which alpha-tocopherol was incorporated into the liposomal membrane, and ascorbate was trapped within the vesicles. Extravesicular ferricyanide was reduced by ascorbate-derived electrons, and this was enhanced by the presence of alpha-tocopherol in the lipid bilayer. When a water-soluble free radical initiator was added to the outside of liposomes, intravesicular ascorbate prevented oxidation of alpha-tocopherol, and this effect was associated with complete protection against peroxidation of membrane lipids. These results suggest that ascorbate-dependent recycling of alpha-tocopherol can protect biological membranes from peroxidation by oxidants originating across the membrane bilayer from ascorbate.
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