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A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator
1Scripps Institution of Oceanography, Marine Biology Research Division, University of California at San Diego, 92093, USA.
Gene
|April 21, 1997
Abstract:
The bacterial cloning vector, pGreenscript A, derived from the mutated Aequorea green fluorescent protein (GFP-S65A) gene, when expressed in E. coli produced colonies that showed yellow color under daylight and strong green fluorescence under long-wave ultraviolet light. The vector was used to select for inserted foreign genes based on the loss of the yellow color/green fluorescence of E. coli cells caused by the insertional inactivation of GFP production.