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Integration of HTLV-1 provirus into mouse transforming growth factor-alpha gene
1Department of Biochemistry and Molecular Oncology, University of Tsukuba, Ibaraki, Japan.
Biochemical and Biophysical Research Communications
|April 28, 1997
Abstract:
Human T cell leukemia virus type 1 (HTLV-1) provirus integration was investigated in mice inoculated with MT-2 cells, a HTLV-1 producing human T-cell line. From spleen DNA derived from a MT-2 cell-injected mouse, we cloned a HTLV-1 integration site by ligation-mediated PCR. The nucleotide sequence showed that HTLV-1 provirus was integrated into an intron of the mouse transforming growth factor-alpha gene in the reverse orientation. This result provides for the first time molecular evidence that mice can be infected with HTLV-1.