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Related Experiment Videos

Alternate COX-2 transcripts are differentially regulated: implications for post-transcriptional control

R Newton1, J Seybold, S F Liu

  • 1Department of Thoracic Medicine, National Heart and Lung Institute, Imperial College School of Medicine, London, United Kingdom.

Biochemical and Biophysical Research Communications
|May 8, 1997
PubMed
Summary

Interleukin-1 beta (IL-1 beta) influences cyclooxygenase-2 (COX-2) gene expression through alternative polyadenylation, affecting mRNA stability and inflammatory responses.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Immunology

Background:

  • Prostaglandin synthesis is crucial during inflammation, primarily regulated by cyclooxygenase-2 (COX-2).
  • COX-2 expression is transcriptionally controlled, but post-transcriptional mechanisms may also play a role.

Purpose of the Study:

  • To investigate the role of post-transcriptional regulation in controlling COX-2 expression.
  • To identify and characterize different COX-2 mRNA transcripts and their regulation by IL-1 beta.

Main Methods:

  • Northern analysis and anchored PCR were used to identify and analyze COX-2 mRNA transcripts in A549 cells.
  • Sequence analysis was performed on amplified cDNA products.
  • Luciferase reporter assays were employed to study the function of the COX-2 3'-untranslated region (UTR).

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  • In vivo studies in LPS-treated rats were conducted to observe differential gene expression.

    • Main Results:

    • Multiple IL-1 beta-inducible COX-2 mRNA transcripts were identified, with predominant 4.5 kb and 2.7 kb forms.
    • These transcripts result from alternative polyadenylation site usage.
    • The 4.5 kb COX-2 mRNA exhibited a half-life exceeding two hours and was stabilized by IL-1 beta.
    • The COX-2 3'-UTR conferred partial IL-1 beta responsiveness in reporter gene assays.
    • Similar differential expression of COX-2 mRNA isoforms was observed in LPS-treated rats.

    Conclusions:

    • Post-transcriptional mechanisms, particularly alternative polyadenylation, are important in regulating COX-2 expression.
    • These mechanisms contribute to the control of COX-2 mRNA levels and stability during inflammation.
    • Findings suggest a complex regulatory network involving both transcriptional and post-transcriptional control of COX-2.