Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protooncogene expression in osteogenesis induced by bone morphogenetic protein

S Sakano1, Y Murata, H Iwata

  • 1Department of Orthopaedic Surgery, Nagoya University School of Medicine, Japan.

Clinical Orthopaedics and Related Research
|May 1, 1997
PubMed
Summary

Protooncogenes c-fos and c-myc messenger RNA expression increased during bone morphogenetic protein-induced bone and cartilage development in mice. These genes showed elevated levels before calcification, indicating their role in early chondroosteogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence for the covalent binding of SHAP, heavy chains of inter-alpha-trypsin inhibitor, to hyaluronan.

The Journal of biological chemistry·1995
Same author

[Clean intermittent catheterization in neurogenic bladder patients with vesicoureteral reflux].

Nihon Hinyokika Gakkai zasshi. The japanese journal of urology·1995
Same author

[A trial of antegrade perfusion to the lower half of the body with selective cerebral perfusion for repair of aortic arch aneurysm combined with atherosclerosis obliterance].

Kyobu geka. The Japanese journal of thoracic surgery·1995
Same author

Inhibition of intestinal tumor development in rat multi-organ carcinogenesis and aberrant crypt foci in rat colon carcinogenesis by 22-oxa-calcitriol, a synthetic analogue of 1 alpha, 25-dihydroxyvitamin D3.

Carcinogenesis·1995
Same author

Varus derotation osteotomy for persistent dysplasia in congenital dislocation of the hip. Proximal femoral growth and alignment changes in the leg.

The Journal of bone and joint surgery. British volume·1995
Same author

Effects of chondroitinase ABC on degenerative intervertebral discs.

Clinical orthopaedics and related research·1995

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Protooncogenes, such as c-fos and c-myc, play critical roles in cellular proliferation and differentiation.
  • Bone morphogenetic proteins (BMPs) are key signaling molecules in bone and cartilage formation.
  • Understanding the temporal expression of protooncogenes during BMP-induced osteogenesis is crucial for regenerative medicine.

Purpose of the Study:

  • To investigate the dynamic changes in c-fos and c-myc messenger RNA expression during bone morphogenetic protein-induced chondroosteogenesis in mice.
  • To correlate protooncogene expression patterns with cellular events during bone and cartilage development.

Main Methods:

  • Quantitative analysis of c-fos and c-myc messenger RNA expression in mouse models following bone morphogenetic protein implantation.

Related Experiment Videos

  • Histological examination of cellular aggregation, mesenchymal cell proliferation, and chondroblast appearance.
  • Time-course analysis of gene expression from Day 1 to Day 11 post-implantation.

    • Main Results:

    • c-fos expression exhibited a biphasic pattern, with initial increases on Day 1 (cell aggregation) and Day 7 (chondroblast appearance).
    • c-myc messenger RNA levels remained elevated from Day 2 to Day 7, coinciding with significant mesenchymal cell proliferation.
    • Both c-fos and c-myc expression decreased after Day 11, despite ongoing chondroosteogenesis.

    Conclusions:

    • Protooncogenes c-fos and c-myc expression are upregulated prior to calcification in BMP-induced bone and cartilage development.
    • The temporal expression of these protooncogenes suggests their involvement in the early stages of mesenchymal cell proliferation and differentiation during osteogenesis.
    • These findings provide insights into the molecular mechanisms governing BMP-mediated skeletal development.