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Related Experiment Videos

Determination of selenium in feeds and premixes: collaborative study

I S Palmer1, N Thiex

  • 1South Dakota State University, Department of Chemistry and Biochemistry, Brookings 57007, USA.

Journal of AOAC International
|May 1, 1997
PubMed
Summary

This study validated two methods for determining selenium (Se) in animal feed: fluorometric and hydride generation atomic absorption (HGAA). Both methods were adopted by AOAC INTERNATIONAL, indicating their reliability for feed analysis.

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Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Food Science

Background:

  • Accurate determination of selenium (Se) is crucial for animal nutrition and health.
  • Existing methods for Se analysis in feed require validation for reliability and reproducibility.
  • Collaborative studies are essential for establishing standardized analytical procedures.

Purpose of the Study:

  • To evaluate the performance of fluorometric and continuous hydride generation atomic absorption (HGAA) methods for selenium determination in animal feeds and premixes.
  • To compare the repeatability and reproducibility of the two analytical methods.
  • To facilitate the adoption of validated methods by regulatory bodies like AOAC INTERNATIONAL.

Main Methods:

  • A collaborative study involving 17 laboratories analyzing blind duplicate feed and premix samples.

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  • Utilized fluorometric and continuous hydride generation atomic absorption (HGAA) techniques for selenium quantification.
  • Statistical analysis of data, including repeatability (RSDr) and reproducibility (RSDR) standard deviations.

    • Main Results:

    • Data from 8 laboratories were statistically analyzed after excluding those with invalid results or probable errors.
    • Fluorometric method showed RSDr from 5.9% to 33% and RSDR from 12% to 33%.
    • HGAA method demonstrated RSDr from 2.8% to 18% and RSDR from 4.0% to 36%.

    Conclusions:

    • Both fluorometric and continuous HGAA methods are suitable for determining selenium in feeds and premixes.
    • The study successfully validated these methods, leading to their adoption by AOAC INTERNATIONAL.
    • Standardized methods ensure accurate selenium content assessment in animal feed for nutritional purposes.