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Construction and characterization of Escherichia coli genetically engineered for bioremediation of
1Institute for Comparative and Environmental Toxicology, Cornell University, Ithaca, New York 14853, USA.
Applied and Environmental Microbiology
|June 1, 1997
Abstract:
Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein significantly increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent properties for bioremediation of Hg(2+)-contaminated environments.