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Cloning and sequence analysis of putative histidine protein kinases isolated from Lactococcus lactis MG1363
M O'Connell-Motherway1, G F Fitzgerald, D van Sinderen
1Department of Microbiology, University College, Cork, Ireland.
Applied and Environmental Microbiology
|June 1, 1997
Abstract:
Eight recombinant plasmids harboring chromosomal fragments of Lactococcus lactis MG1363 were shown to phenotypically suppress a histidine protein kinase (HPK) deficiency in either of two different E. coli strains. Sequence analysis of the plasmid inserts revealed five different complete or partial open reading frames (ORFs) specifying proteins with high similarity to HPKs. One of the plasmids also harbored an additional ORF, unrelated to HPKs, with suppressing activity.