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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
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Determination of esterase-catalyzed cocaine metabolite formation by high-performance liquid chromatography with

K Y Kim1, L M Bornheim

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143-0450, USA.

Journal of Chromatographic Science
|June 1, 1997
PubMed

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...