Related Experiment Video
Updated: Jul 28, 2026

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
Published on: August 9, 2011
Optimization of haemolysis in enhanced haemolysis agar (EHA)--a selective medium for the isolation of Listeria
R R Beumer1, M C te Giffel, L J Cox
1Laboratory of Food Microbiology and Hygiene, Agricultural University, Wageningen, The Netherlands.
Abstract:
The presence of Listeria monocytogenes in enrichment media can be masked by faster growth of other Listeria spp. Therefore, enhanced haemolysis agar (EHA) is a good alternative for another isolation media, because the presence of a few L. monocytogenes colonies can be detected in a majority of colonies of other listeriae on the basis of haemolysis. In this study the haemolysis reaction in EHA was optimized. In a collaborative study using reference samples, no significant differences in counts on EHA, Palcam and Oxford agar were shown.

