Related Experiment Video
Updated: Aug 13, 2026

08:27
The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse
Published on: July 25, 2011
[Effect of ischemic damage factors on lipid peroxidation in rat brain synaptosomes]
Biofizika
|March 1, 1997
Abstract:
Accumulation of TBA-reactive substances after a 40 min incubation of rat brain synaptosomes at 37 degrees C was analysed. A lowering of pH to 6.5-5.5 or arachidonic acid (0.1-1.0 mM) increased lipid peroxidation which was blocked by antioxidants. Acidosis (pH 6.0) and arachidonic acid used in combination had a strong synergic effect. Depolarisation of plasma membranes or intrasynaptosomal mitochondria were without influence on lipid peroxidation at neutral or acid pH. The results support a leading role of acidosis and phospholipases in stimulation of peroxidation under ischaemia.

