[The collagen-binding membrane proteins of connective-tissue cells]
Related Experiment Video
Updated: Nov 1, 2026

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
Published on: April 22, 2019
Abstract:
The pattern of cell membrane collagen-binding proteins was analysed by affinity chromatography with collagen types I, III and IV columns. We have found that connective tissue cells have three polypeptides which are able to recognize the types of collagen. These proteins have molecular weights 130, 190, and 250 kDa (reduced) according to SDS-PAGE. Proteins with M. W. 130 and 250 kDa show high affinity to collagen type III and IV, but not to collagen type I. Protein with M. W. 190 kDa binds collagen type I only. The distribution of these proteins between cells from various tissues strongly correlates with the set of collagen types interacting with cells in the intact tissue.
Related Concept Videos
Matrix Proteoglycans and Glycoproteins
Overview of Cell-Matrix Interactions
Collagens are the Major Structural Proteins of ECM
Connective tissue proper includes loose...
The Extracellular Matrix
The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Fibril-associated Collagen
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
