Alpha2B adrenoceptor mRNA expression during rat brain development

The expression of alpha2B adrenoceptor mRNA in developing and adult rat brain was examined, using in situ hybridization with S-labeled riboprobes. In the adult we have detected more widespread expression than previously reported, with moderate to strong hybridization signals in the fundus striati, olfactory tubercles, septum, thalamus and Purkinje cells of the cerebellum. In addition, there was low expression in the endopiriform nucleus, claustrum, cortex, caudate-putamen and spinal trigeminal nucleus of the brain stem. During embryonic development, there was intense, transient mRNA expression in the developing vascular plexus and vasculature which disappeared by birth. In most brain areas which exhibit mRNA expression in the adult, expression started during late embryonic development; with the exception of the thalamus, where expression was differentially regulated in sensory and non-sensory thalamic nuclei starting at the end of the first postnatal week. In addition, there was transiently upregulated expression in the caudate-putamen and cerebellar Purkinje cells during late embryonic and early postnatal development, respectively. This transient expression correlates with the time of neuronal migration and differentiation in these structures and complements the developmental expression of alpha2A and alpha2C adrenoceptors. These results suggest that alpha2B adrenoceptors may play a role in angiogenesis and in mediating neurotrophic functions of norepinephrine in some brain areas.