Profile of glutamylated tubulin expression during cerebellar granule cell development in vitro

The developmental regulation of tubulin and several of its posttranslational modifications was examined during the differentiation of rat cerebellar granule cells in vitro. In particular, we have noted that the glutamylation of alpha- and beta-tubulin subunits varies during development and becomes more prominent in differentiating neuronal processes. These results indicate that glutamylation of tubulin may be important in the stabilization of microtubules during the maturation of the neuronal cytoskeleton.