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Related Experiment Videos

Eccentric contractions decrease glucose transporter transcription rate, mRNA, and protein in skeletal muscle

S Kristiansen1, J Jones, A Handberg

  • 1Copenhagen Muscle Research Centre, August Krogh Institute, Copenhagen, Denmark.

The American Journal of Physiology
|May 1, 1997
PubMed
Summary
This summary is machine-generated.

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Eccentric muscle contractions reduce glucose transporter (GLUT-4) levels in rat skeletal muscle by decreasing gene transcription and mRNA. This leads to less GLUT-4 on the cell surface, impairing glucose uptake during exercise.

Area of Science:

  • Exercise Physiology
  • Molecular Biology
  • Muscle Metabolism

Background:

  • Eccentric contractions (ECs) cause a delayed decrease in glucose transporter (GLUT-4) protein and muscle glucose transport.
  • The underlying molecular mechanisms, particularly changes in gene expression, remain to be fully elucidated.

Purpose of the Study:

  • To investigate if prior ECs alter GLUT-4 gene transcription and mRNA levels in rat skeletal muscle.
  • To determine the impact of ECs on sarcolemmal GLUT-4 protein content in response to subsequent muscle stimulation.

Main Methods:

  • Rats underwent electrically stimulated ECs, with contralateral unstimulated muscle serving as control.
  • GLUT-4 gene transcription rate (nuclear run-on), GLUT-4 mRNA levels, and total/sarcolemmal GLUT-4 protein content were measured 2 days post-ECs.

Related Experiment Videos

  • Plasma membrane (PM) vesicles were isolated from stimulated muscles to assess sarcolemmal GLUT-4.

    • Main Results:

    • Two days after ECs, total GLUT-4 protein decreased by 32-50% and GLUT-4 mRNA by 41% in rat gastrocnemius muscle.
    • The GLUT-4 transcription rate was significantly reduced by 75% in EC muscle compared to controls.
    • Sarcolemmal GLUT-4 protein content in PM vesicles from stimulated EC muscle was reduced by 51%.

    Conclusions:

    • ECs decrease skeletal muscle GLUT-4 transcription rate and mRNA levels, leading to reduced total GLUT-4 protein.
    • This reduction in GLUT-4 ultimately diminishes the translocation of transporters to the sarcolemma during contraction.
    • Consequently, ECs impair contraction-stimulated muscle glucose transport due to decreased GLUT-4 availability.