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Related Experiment Videos

Gastric epithelial morphogenesis in normal and transgenic mice

S M Karam1, Q Li, J I Gordon

  • 1Department of Anatomy, Faculty of Medicine, Kuwait University, Safat, Kuwait.

The American Journal of Physiology
|May 1, 1997
PubMed
Summary

Parietal cells guide gastric unit development by signaling to other cell types. This study reveals parietal cell lineage instructions influence neck-zymogenic cells early in stomach development.

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Area of Science:

  • Developmental Biology
  • Gastroenterology
  • Cell Lineage Specification

Background:

  • Gastric units in the adult mouse stomach epithelium are formed by pit, parietal, and neck-zymogenic cell lineages originating from multipotent stem cells.
  • Understanding the precise developmental trajectory and intercellular communication during gastric unit morphogenesis is crucial for comprehending stomach development and potential pathologies.

Purpose of the Study:

  • To investigate the process of gastric unit morphogenesis from embryonic day 18 to postnatal day 28 in mice.
  • To elucidate the role of parietal cells in regulating the development and differentiation of other cell lineages within the gastric unit.

Main Methods:

  • Utilized electron microscopy and multilabel immunohistochemistry to examine gastric unit development in normal FVB/N mice.

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  • Employed a transgenic approach using simian virus 40 large T antigen (SV40 TAg) driven by the H(+)-K(+)-adenosinetriphosphatase beta-subunit gene promoter to specifically target the parietal cell lineage.

    • Main Results:

    • Gastric units develop from solid primordial buds into elongated structures with distinct cell lineages and a proliferative isthmus by postnatal day 21.
    • Targeting parietal cell lineage with SV40 TAg led to an expansion of pre-parietal cells and precursors, a block in parietal cell differentiation, and a decrease in neck and zymogenic cells.
    • Evidence suggests parietal cell lineage members provide regulatory signals to the neck-zymogenic cell lineage even at early developmental stages.

    Conclusions:

    • Parietal cell lineage plays a critical instructive role in the coordinated development of the gastric unit.
    • Interactions originating from the parietal cell lineage influence the differentiation and abundance of neck-zymogenic cells prior to the full compartmentalization of the gastric unit.