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Related Experiment Videos

Thyrotropin receptor transcripts in human adipose tissue

M S Crisp1, C Lane, M Halliwell

  • 1Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, UK.

The Journal of Clinical Endocrinology and Metabolism
|June 1, 1997
PubMed
Summary
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Thyroid associated ophthalmopathy (TAO) involves the thyrotropin receptor (TSHR). TSHR transcripts are found in normal orbital fat but are significantly elevated in TAO orbital fat, suggesting a role in the condition.

Area of Science:

  • Endocrinology
  • Immunology
  • Ophthalmology

Background:

  • Thyroid associated ophthalmopathy (TAO) is an autoimmune condition linked to Graves' disease.
  • The thyrotropin receptor (TSHR) is a suspected target antigen in TAO.
  • Previous research on TSHR transcripts in the orbit has yielded inconsistent findings.

Purpose of the Study:

  • To investigate the presence and levels of TSHR transcripts in orbital adipose and muscle tissues.
  • To determine if TSHR expression differs between normal and TAO orbital tissues.

Main Methods:

  • RT-PCR amplification of TSHR transcripts from human adipose and muscle tissues.
  • Northern blot analysis to detect and quantify TSHR transcripts.
  • Comparison of TSHR transcript levels in normal versus TAO orbital fat.

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Main Results:

  • TSHR transcripts were detected in normal intestinal and orbital fat, but not skeletal muscle.
  • Northern blots showed TSHR transcripts at the limit of detection in normal orbital and intestinal fat.
  • Significantly elevated TSHR transcripts (4.6 and 1.7kb) were observed in orbital fat from TAO patients.

Conclusions:

  • Normal adult adipose tissues express low levels of TSHR transcripts.
  • Elevated TSHR transcripts in TAO orbital fat may be due to an increased number of cells, particularly preadipocytes.
  • These findings support TSHR's role in the pathogenesis of TAO.