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Related Experiment Videos

Long-lifetime metal-ligand pH probe

Z Murtaza1, Q Chang, G Rao

  • 1Department of Biochemistry & Molecular Biology, University of Maryland at Baltimore, School of Medicine 21201, USA.

Analytical Biochemistry
|May 1, 1997
PubMed
Summary

A novel pH-sensitive ruthenium complex, [Ru(deabpy)(bpy)2]2., exhibits fluorescence changes near physiological pH. This metal-ligand complex enables lifetime-based sensing and ratiometric probes for point-of-care applications.

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ADVANCES IN FLUORESCENCE SPECTROSCOPY: MULTI-PHOTON EXCITATION, ENGINEERED PROTEINS, MODULATION SENSING AND MICROSECOND RHENIUM METAL-LIGAND COMPLEXES.

Acta physica Polonica: A·2019

Area of Science:

  • Coordination Chemistry
  • Photophysics
  • Chemical Sensing

Background:

  • Metal-ligand complexes (MLCs) are crucial in developing advanced sensors.
  • pH-sensitive probes are vital for physiological and diagnostic applications.
  • Ruthenium complexes offer unique photophysical properties for sensing.

Purpose of the Study:

  • To synthesize and characterize a novel pH-sensitive ruthenium-based metal-ligand complex.
  • To evaluate the complex's potential for lifetime-based and wavelength-ratiometric pH sensing.
  • To explore its utility in physiological pH ranges and point-of-care diagnostics.

Main Methods:

  • Synthesis of the [Ru(deabpy)(bpy)2]2+ complex, where deabpy is 4,4'-diethylaminomethyl-2,2'-bipyridine.
  • Fluorescence spectral characterization and lifetime measurements across a range of pH values.

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  • Phase-modulation fluorometry using amplitude-modulated laser or blue-light-emitting diode.

    • Main Results:

    • The MLC displayed pH-dependent intensity, emission spectra, and decay times centered around pH 7.5.
    • Apparent pKa values were independent of ionic strength.
    • Two distinct decay times (235 ns and 380 ns) were observed for protonated and unprotonated forms, suitable for phase-modulation fluorometry at 700 kHz.
    • A modest spectral shift with pH change allowed for wavelength-ratiometric sensing.

    Conclusions:

    • The synthesized ruthenium complex is a robust pH sensor with distinct optical responses near physiological pH.
    • Its long decay time and suitability for phase-modulation fluorometry facilitate low-frequency optical measurements.
    • The complex shows promise for developing lifetime-based and ratiometric sensors for blood cations and point-of-care assays.