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Antibiotic resistance in Streptomyces lividans: fluorescence assay for streptogramin B lyase
K P Bateman1, S M Armstrong, R L White
1Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
Analytical Biochemistry
|June 1, 1997
Abstract:
A fluorescence assay for streptogramin B lyase, an enzyme that confers resistance to streptogramin B antibiotics, has been developed. The antibiotic substrates are fluorescent and the linear peptide products formed in the lyase-catalyzed reaction are relatively nonfluorescent. The assay has potential for assessing bacterial resistance to streptogramin B antibiotics and will be utilized to direct the purification of streptogramin B lyase from bacterial extracts.