Related Experiment Videos
DNA binding and cleavage by a cationic manganese porphyrin-peptide nucleic acid conjugate
P Bigey1, S H Sönnichsen, B Meunier
1Laboratoire de Chimie de Coordination du CNRS, Toulouse, France.
Bioconjugate Chemistry
|May 1, 1997
Abstract:
A cationic manganese porphyrin-peptide nucleic acid (PNA) conjugate has been prepared and used to cleave a double-stranded DNA target. Cleavage experiments were performed with a 247-base pair restriction DNA fragment containing a 10-base pair homopurine binding target for the PNA. Oxidative activation by this Mn porphyrin-PNA conjugate leads to sequence specific, 3'-staggered cleavage of both DNA strands near the strand displacement junction. Furthermore, the Mn porphyrin-PNA porphyrin conjugates bind over 100-fold better to double-stranded DNA compared to the native PNA.