Potency testing of tissue culture rinderpest vaccine in rabbits

Rinderpest (RP) vaccine potency testing requires virulent bovine rinderpest virus (RPV). Use of virulent RPV is a biosafety hazard. In this study we had vaccinated rabbits with tissue culture RP vaccine at different doses and thereafter challenged with lapinized virus. No thermal reaction in vaccinated rabbits was observed. Serum neutralizing antibody response to vaccine was dose dependent until the second week post-vaccination but by the fourth week post-vaccination all the rabbits had similar neutralizing antibody titres. Vaccinated rabbits exhibited mild clinical signs as compared to unvaccinated controls after challenge. All the vaccinated rabbits survived challenge while only 40% unvaccinated rabbits survived challenge with virulent lapinized RPV. A strong anamnestic response in all the vaccinated rabbits was observed after challenge with lapinized virus. This study shows that rabbits could be used for potency testing of RP vaccine virus.