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Related Experiment Videos

Genotypic variation among different phenotypes within aphid clones

G Lushai1, H D Loxdale, C P Brookes

  • 1Department of Biology, Imperial College at Silwood Park, Ascot, Berkshire, UK.

Proceedings. Biological Sciences
|May 22, 1997
PubMed
Summary
This summary is machine-generated.

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Genetic differences exist between winged and wingless aphids within the same clone. Randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) revealed distinct DNA profiles, challenging the assumption of genetic uniformity in parthenogenetic aphid lineages.

Area of Science:

  • Entomology
  • Genetics
  • Molecular Biology

Background:

  • Aphids (Hemiptera: Aphididae) exhibit parthenogenesis and polyphenism, producing distinct adult morphs (e.g., winged, wingless) within asexual lineages.
  • It is generally assumed that offspring from parthenogenetic aphid clones are genetically identical, barring sexual reproduction.
  • Understanding genetic variation within clonal lineages is crucial for studying population dynamics and evolutionary processes in these insects.

Purpose of the Study:

  • To investigate potential genetic differences between winged and wingless morphs within asexual clones of cereal aphids.
  • To determine if molecular markers can distinguish between different aphid morphs originating from the same clone.
  • To explore the implications of observed genetic variation for the understanding of clonal organisms.

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Main Methods:

  • Randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was employed to analyze DNA from different aphid morphs.
  • Southern blotting and hybridization were used to confirm the origin of polymorphic DNA bands.
  • Sequencing of specific DNA bands and analysis of genomic DNA treated to remove secondary structures were performed.

Main Results:

  • RAPD-PCR revealed the presence or absence of specific DNA bands differentiating winged and wingless morphs in some clones of Sitobion avenae and Rhopalosiphum padi.
  • Southern blotting confirmed that the discriminating DNA bands were of aphid origin.
  • Sequenced bands were A/T rich, with one showing homology to muscle tissue, and differences persisted even after DNA structural modification.

Conclusions:

  • Contrary to assumptions, genetic variations can exist between asexual morphs within the same aphid clone.
  • RAPD-PCR is a viable method for detecting these subtle genetic distinctions.
  • This finding has significant implications for understanding genetic identity and variation in clonal organisms like aphids.