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Related Experiment Videos

On the role of calcium in the regulatory volume decrease (RVD) response in Ehrlich mouse ascites tumor cells

N K Jorgensen1, S Christensen, H Harbak

  • 1Biochemical Department, The August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen O, Denmark.

The Journal of Membrane Biology
|June 1, 1997
PubMed
Summary

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Calcium signaling does not activate the regulatory volume decrease (RVD) response in Ehrlich cells. Studies found no evidence of calcium (Ca2+) release or entry influencing this crucial cell volume regulation mechanism.

Area of Science:

  • Cell Biology
  • Physiology
  • Biochemistry

Background:

  • The regulatory volume decrease (RVD) response is vital for cell survival under hypotonic stress.
  • Calcium ions (Ca2+) are often implicated in cellular signaling pathways, including volume regulation.

Purpose of the Study:

  • To investigate the role of Ca2+ entry and mobilization in activating the RVD response in Ehrlich cells.
  • To determine if Ca2+ signaling pathways are involved in the volume-induced K+ loss during RVD.

Main Methods:

  • Hypotonic exposure of Ehrlich cells (50% osmolarity).
  • Measurement of inositol trisphosphate (Ins(1,4,5)P3) content.
  • Ca2+ signaling assessment using fura-2 loaded cells and fluorescence-ratio imaging.
  • Monitoring of K+ loss and RVD response.

Related Experiment Videos

  • Use of Ca2+ chelator BAPTA and Ba2+ as a Ca2+ substitute.

    • Main Results:

    • No increase in Ins(1,4,5)P3 content was observed.
    • No significant Ca2+ signaling was detected in cell suspensions or single cells.
    • External Ca2+ removal did not affect volume-induced K+ loss.
    • BAPTA loading did not inhibit the RVD response.
    • Ba2+ partially inhibited swelling-induced K+ loss, but specific inhibitors of Ca2+-mobilizing agonists had minimal effect.

    Conclusions:

    • Ca2+ signaling, whether through release or entry, does not appear to play a role in the activation of the RVD response in Ehrlich cells.
    • The RVD mechanism in Ehrlich cells operates independently of Ca2+ signaling pathways.