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Th1 CD4+ cells adoptively transfer experimental hypersensitivity pneumonitis
1Albuquerque VA Medical Center, University of New Mexico School of Medicine, The Lovelace Institutes, 87108, USA.
Cellular Immunology
|May 1, 1997
Summary
Th1 CD4+ cells adoptively transfer hypersensitivity pneumonitis (EHP) in mice, while Th2 cells do not. This transfer capability correlates with IFN-gamma production and inversely with IL4 secretion, highlighting Th1 cells
Area of Science:
- Immunology
- Cell Biology
Background:
- Murine experimental hypersensitivity pneumonitis (EHP) can be adoptively transferred by cultured cells from sensitized donors.
- CD4+ T cells play a crucial role in the immune response, differentiating into distinct subsets like Th1 and Th2.
Purpose of the Study:
- To determine if CD4+ T cells mediating EHP transfer exhibit Th1 or Th2 characteristics.
- To establish and characterize Th1 and Th2 cell lines from M. faeni-sensitized mice.
Main Methods:
- Established CD4+ T cell lines from lung-associated lymph nodes of M. faeni-sensitized mice using antigen and specific cytokine cocktails (IFN-gamma/IL2/anti-IL4 for Th1, IL4 for Th2).
- Adoptively transferred cultured cell lines into recipient mice, followed by intratracheal challenge with M. faeni to assess EHP development.
- Quantified cytokine secretion (IFN-gamma, IL4, IL10) to confirm Th1/Th2 differentiation.
Main Results:
- Differentiated Th1 cell lines produced high IFN-gamma and low IL4/IL10, while Th2 cell lines produced high IL4/IL10 and low IFN-gamma.
- Only Th1 CD4+ cell lines were capable of adoptively transferring EHP.
- EHP transfer was directly proportional to IFN-gamma levels and inversely proportional to IL4 levels.
Conclusions:
- It is possible to generate distinct Th1 and Th2 CD4+ cell lines from M. faeni-sensitized mice.
- Th1 CD4+ cells are essential for the adoptive transfer of murine experimental hypersensitivity pneumonitis.