Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polypeptide chain release factors

R H Buckingham1, G Grentzmann, L Kisselev

  • 1CNRS UPR9073, Institut de Biologie Physico-chimique, Paris, France. rhb@ibpc.fr

Molecular Microbiology
|May 1, 1997
PubMed
Summary

Termination factors like RF3 and eRF3 are crucial for releasing new polypeptide chains from ribosomes. Recent advances clarify protein synthesis termination, but mechanisms of these GTP-hydrolysing factors remain under investigation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial polypeptide release factor RF2 is structurally distinct from eukaryotic eRF1.

Molecular cell·2002
Same author

A posttermination ribosomal complex is the guanine nucleotide exchange factor for peptide release factor RF3.

Cell·2001
Same author

Class-1 translation termination factors: invariant GGQ minidomain is essential for release activity and ribosome binding but not for stop codon recognition.

Nucleic acids research·2001
Same author

Cleavage of colicin D is necessary for cell killing and requires the inner membrane peptidase LepB.

Molecular cell·2001
Same author

Stop codon recognition in ciliates: Euplotes release factor does not respond to reassigned UGA codon.

EMBO reports·2001
Same author

Mg2+-induced tRNA folding.

Biochemistry·2001

Area of Science:

  • Molecular Biology
  • Protein Synthesis
  • Genetics

Background:

  • Protein synthesis termination involves releasing polypeptide chains from peptidyl-tRNA upon encountering mRNA stop signals.
  • Extra-ribosomal proteins, known as release factors, are essential for this termination process.
  • Despite decades of study since the 1960s, the precise mechanisms of protein synthesis termination remain incompletely understood.

Purpose of the Study:

  • To review recent significant advances in understanding protein synthesis termination in both prokaryotic and eukaryotic organisms.
  • To highlight remaining areas of uncertainty in the termination process.
  • To focus on proposed models for the action of GTP-hydrolysing termination factors, specifically RF3 and eRF3.

Main Methods:

  • Literature review of recent research on protein synthesis termination.
  • Analysis of proposed models for termination factor action.
  • Comparative study of termination mechanisms in prokaryotes and eukaryotes.

Main Results:

  • Significant progress has been made in elucidating the molecular mechanisms of protein synthesis termination.
  • Key GTP-hydrolysing termination factors, RF3 (prokaryotes) and eRF3 (eukaryotes), have been investigated.
  • Specific models for the action of these factors have been proposed, though uncertainties persist.

Conclusions:

  • Recent research has substantially advanced our understanding of protein synthesis termination.
  • The roles and mechanisms of RF3 and eRF3 in termination require further investigation.
  • Continued research is vital to fully unravel the complexities of this fundamental biological process.

Related Experiment Videos