[Covalent binding of acylated trypsin with polymeric carriers]

In order to provide covalent binding of trypsin with polymer carriers, trypsin was activated by an administration of double C=C bond. Thereafter, activated trypsin was polymerized together with a monomer of the acrylic series. Trypsin acylation with an unsaturated acid allowed the enzyme immobilization by means of its copolymerization with a monomer forming a polymer matrix. The specific proteolytic activity of immobilized trypsin decreased in proportion to an increase in the molecular weight of the polymerization product.