Related Experiment Videos
Surface plasmon resonance analysis of topoisomerase I-DNA binding: effect of Mg2+ and DNA sequence
C D Pond1, J A Holden, P C Schnabel
1Department of Pharmacology, University of Utah, Salt Lake City 84112, USA.
Anti-Cancer Drugs
|April 1, 1997
Abstract:
Surface plasmon resonance detection was used to characterize interactions of human topoisomerase I and DNA. The disassociation of topoisomerase I and DNA is characterized by two rate constants. This suggests two parallel but independent pathways of release. DNA association appears to be complex. Mg2+ was found to increase both disassociation rate constants and may have a detectable effect on topoisomerase I-DNA association. DNA base content did not affect disassociation rate constants or the rate of association.