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A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Study of hepatitis E virus replication in FRhK-4 cell culture]
Abstract:
The cytolytic form of hepatitis E virus reproduction in FRhk-4 cells is described. The cytopathic effect was observed by days 6-7 of the 25th passage in this cell culture. Reverse transcription with polymerase chain reaction were used to study virus reproduction daily for 10 days postinfection. The virus replication starts on day 4 after inoculation. The development of the cytopathic effect depended on the serum concentration in culture medium, whereas the virus replication did not depend on this factor. The replicative form (-RNA) of viral RNA appeared on day 4 postinoculation, as did the new genomic one (+RNA).
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