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Related Experiment Videos

Fission yeast dim1(+) encodes a functionally conserved polypeptide essential for mitosis

L D Berry1, K L Gould

  • 1Howard Hughes Medical Institute, Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37212, USA.

The Journal of Cell Biology
|June 16, 1997
PubMed
Summary

The novel dim1-35 mutant reveals Dim1p is essential for mitosis entry and chromosome segregation in fission yeast. This protein is conserved across species and may function in mitotic spindle assembly.

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Study of cyclin proteolysis in anaphase-promoting complex (APC) mutant cells reveals the requirement for APC function in the final steps of the fission yeast septation initiation network.

Molecular and cellular biology·2001

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • The cell cycle regulation is crucial for proper cell division.
  • Mitosis involves precise chromosome segregation and nuclear division.
  • Understanding genes controlling these processes is vital for cell biology.

Purpose of the Study:

  • To identify new genes involved in cell cycle control.
  • To characterize the function of the novel gene Dim1.
  • To investigate the role of Dim1 in mitosis and chromosome segregation.

Main Methods:

  • Screening for suppressors of a fission yeast mutant.
  • Isolation and characterization of temperature-sensitive mutants (dim1-35).
  • Gene deletion and complementation studies in yeast and mouse models.

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  • Analysis of cell cycle progression, histone H1 kinase activity, and drug sensitivity.

    • Main Results:

    • A novel temperature-sensitive mutant, dim1-35, was identified.
    • dim1-35 mutants exhibit defects in mitosis entry and chromosome segregation.
    • Deletion of Dim1 is lethal, causing G2 arrest, rescued by homologs.
    • dim1-35 shows low H1 kinase activity and sensitivity to microtubule-destabilizing drugs.

    Conclusions:

    • Dim1p plays a fundamental, conserved role in mitosis entry and chromosome segregation.
    • Dim1p is essential for cell viability, likely involved in mitotic spindle function.
    • The findings highlight Dim1p's importance in maintaining genomic stability during cell division.