Interleukin-17 up-regulation of nitric oxide production in human osteoarthritis cartilage
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M G Attur1, R N Patel, S B Abramson
1Hospital for Joint Diseases, New York, New York 10003, USA.
Objective:
To examine the effect of human interleukin-17 (IL-17) on nitric oxide (NO) production in human osteoarthritis (OA) cartilage under ex vivo conditions.
Methods:
OA cartilage from patients undergoing knee replacement surgery was used in explant assays to assess the effect of IL-17. NO production was measured by estimating the stable NO metabolite, nitrite, in conditioned medium.
Results:
IL-17 augmented the spontaneous production of nitric oxide. This augmentation was sensitive to cycloheximide and pyrrolidine dithiocarbamate, but not to dexamethasone or soluble IL-1 receptor.
Conclusion:
IL-17 augments nitric oxide production in OA cartilage via nuclear factor kappaB activation, but independently of IL-1beta signaling.