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Related Experiment Videos

Human cells and cell cultures: availability, authentication and future prospects

R J Hay1

  • 1American Type Culture Collection (ATCC), Rockville, MD 20852, USA. rhay@atcc.org

Human Cell
|September 1, 1996
PubMed
Summary

High-quality human cells and cell lines are crucial for biomedical research and transplantation. Advances in cell banking and culture techniques are improving availability and comparability for scientific study.

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Area of Science:

  • Biomedical research
  • Cell biology
  • Tissue engineering

Background:

  • Well-characterized human cells, tissues, and cell lines are essential for transplantation and biomedical research.
  • Existing networks in the USA provide primary human cells and tissues, but face challenges like donor privacy, supply limitations, and regulatory hurdles.
  • Human cell lines offer advantages over primary tissues, overcoming many supply and logistical issues.

Purpose of the Study:

  • To highlight the importance of well-characterized human cells, tissues, and cell lines for research.
  • To discuss the challenges and solutions in providing these biological materials.
  • To emphasize the role of cell banks and advancements in cell culture for future research.

Main Methods:

  • Review of existing institutional and multi-center networks for human cell and tissue provision.

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  • Discussion of challenges including donor rights, transport, survival, data correlation, and contamination.
  • Exploration of cell line utilization, national cell banks, and authentication procedures.
  • Examination of modern techniques like improved culture ware, serum-free media, and growth factors.
  • Consideration of hybridization and immortalization techniques for cell propagation.

    • Main Results:

    • Established networks in the USA facilitate the provision of human cells and tissues.
    • Human cell lines, supported by national cell banks, enhance research comparability and overcome supply limitations.
    • Rigorous authentication, including histological, biochemical, immunological, and microbial/viral testing, is critical for both tissues and cell lines.
    • Modern cell culture techniques and immortalization strategies are expanding the availability of diverse human cell types for research.

    Conclusions:

    • The availability of well-characterized human cells, tissues, and cell lines is fundamental to advancing biomedical research and transplantation.
    • Cell banking, rigorous authentication, and innovative culture techniques are vital for ensuring the quality and comparability of research materials.
    • Future advancements promise broader accessibility to a wide range of human cell types for scientific study.