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Identification of beta-adrenergic receptor subtypes mediating relaxation in isolated equine ileum
Objective:
To identify beta-adrenergic receptor subtypes in ileum smooth muscle of the horse.
Sample Population:
Isolated strips of equine longitudinal ileum smooth muscle and membrane preparations from smooth muscle of the intestinal wall.
Procedure:
Functional assays and radioligand binding assays.
Results:
Relaxation of ileum longitudinal smooth muscle proved to be mainly caused by stimulation of beta-atypical and beta 2-adrenergic receptors. Binding studies on cell membranes indicated that the total beta-adrenergic receptors population consists of 54% beta-atypical, 34% beta 2- and 12% beta 1-subtypes.
Conclusions:
The data suggest that sympathetic relaxation of equine ileum smooth muscle depends mainly on beta-atypical receptor subtypes activation, with a minor contribution by beta 2-subtypes.
Clinical Relevance:
The important role of beta-atypical adrenergic receptor subtypes in the relaxation of equine ileum suggests possible clinical use of selective beta-atypical receptor agonists to control intestinal disturbances.