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Apoptotic condensations in M-phase cells

K H Sit1, L Yin, R Paramanantham

  • 1Department of Anatomy, Faculty of Medicine, National University of Singapore, Kent Ridge, Singapore.

The Anatomical Record
|June 1, 1997
PubMed
Summary
This summary is machine-generated.

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This study demonstrates that M-phase cells can exhibit apoptotic morphologies, challenging the notion of mutual exclusivity between mitosis and apoptosis. Oxidative stress or endonuclease activity can induce these programmed cell death features in dividing cells.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Apoptosis, or programmed cell death, is characterized by DNA fragmentation and chromatin condensation.
  • Traditionally, apoptosis and mitosis (cell division) were considered mutually exclusive processes.
  • M-phase cells were not thought to display apoptotic features.

Purpose of the Study:

  • To investigate whether M-phase cells can exhibit apoptotic morphologies.
  • To determine if oxidative stress or endonuclease activity can induce apoptosis in mitotic cells.

Main Methods:

  • Human Chang liver cells were subjected to oxidative stress using vanadyl(4) or treated with micrococcal nuclease.
  • DNA fragmentation was assessed using propidium iodide staining and TUNEL assay.

Related Experiment Videos

  • Apoptotic morphologies were visualized via Giemsa and neutral red staining, and flow cytometry.

    • Main Results:

    • Apoptotic morphologies, including DNA fragmentation and chromatin condensation, were observed in M-phase cells (prophase, metaphase, anaphase, telophase).
    • Both oxidative stress and direct endonuclease digestion induced these apoptotic features in dividing cells.
    • Observed chromatin changes in M-phase cells may explain micronuclei formation.

    Conclusions:

    • M-phase cells can undergo apoptosis.
    • Apoptotic morphologies in M-phase cells can be induced by oxidative stress or endonuclease activity.
    • This finding challenges the traditional view of apoptosis and mitosis as mutually exclusive.