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Electromotive administration of oxybutynin into the human bladder wall
S M Di Stasi1, A Giannantoni, R Massoud
1Department of Surgery/Urology, Tor Vergata University of Rome School of Medicine, Italy.
Purpose:
To compare concentrations of oxybutynin in the human bladder wall after either passive delivery (PD) or electromotive administration (EMDA).
Materials And Methods:
Tissue sections of human bladder were inserted into a diffusion cell with urothelium exposed to the donor compartment containing oxybutynin (4.5 mg. in 100 ml. NaCl 0.45%) and an anode. Twelve paired experiments, "current 5 mA/no current", were conducted over 15 minutes. Oxybutynin tissue contents were measured and tissue viability, morphology and oxybutynin stability were assessed.
Results:
Mean oxybutynin tissue concentrations were 3.84 micrograms./gm. in samples exposed to EMDA and 0.87 microgram./gm. in samples exposed to PD (p = 0.0006). The mean coefficients of variation were 57.85% in EMDA experiments and 89.78% in PD experiments. Tissues were viable and undamaged histologically and no oxybutynin structural modification was observed.
Conclusions:
EMDA enhances oxybutynin administration into viable bladder wall and reduces the variability in drug delivery rate.