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Generation of small insert genomic FISH probes with high signal intensity suitable for deletion mapping
M H Dreyling1, O I Olopade, S K Bohlander
1Abteilung für Hämatologie und Onkologie, Georg-August-Universität Götingen, Germany. mdreylin@mdv.gwdg.de
Cytogenetics and Cell Genetics
|January 1, 1997
Abstract:
We have developed a method to generate FISH probes from small (2-4 kb) nonrepetitive genomic restriction fragments. The probes showed a high hybridization efficiency of up to 90% in metaphase cells from normal peripheral blood. With the use of these probes, homozygous as well as hemizygous 9p deletions were reliably identified in nine leukemia-derived cell lines.