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Defective tumor vascularization induced by metastasin 1 expression
A Onischenko1, M P Chenard, O Lefebvre
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, Illkirch, France.
Invasion & Metastasis
|January 1, 1996
Summary
Metastasin 1 (mts1) expression in breast cancer cells did not increase invasiveness. However, mts1 expression led to defective tumor microvessels, suggesting a negative impact on angiogenesis.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Metastasin 1 (mts1), an S100 Ca(2+)-binding protein, is implicated in tumor progression.
- Its role in metastasis requires further investigation.
Purpose of the Study:
- To investigate the role of mts1 in tumor progression and metastasis.
- To determine the effect of mts1 expression on MCF7 breast cancer cells.
Main Methods:
- Gene transfer of human sense mts1 into MCF7 cells.
- In vitro assessment of proliferation and invasion.
- In vivo tumor growth and metastasis studies in mice.
- Immunohistochemical analysis of tumor microvasculature.
Main Results:
- Mts1 expression did not alter in vitro proliferation or invasion of MCF7 cells.
- In vivo, mts1-expressing tumors showed necrosis and altered stroma.
- Mts1 expression reduced the number and size of tumor microvessels, with some collapsing.
- No metastases were observed in either group.
Conclusions:
- Mts1 does not confer invasive properties to MCF7 cells.
- Mts1 expression negatively impacts tumor angiogenesis and microvessel maintenance.
- Mts1 may play an inhibitory role in neoangiogenesis.