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New assay for glycated lipoproteins by high-performance liquid chromatography
1Third Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Annals of the New York Academy of Sciences
|April 15, 1997
Abstract:
We developed an automated analytic system for glycated lipoprotein using high-performance liquid chromatography (HPLC) with an affinity boronate column and a gel permeation column. This system can measure glycated lipoprotein (glycated LDL and glycated HDL) in a small (5 microliters) sample of serum in a short time (40 min/sample). Recovery with this system was 92.1%. Therefore a large number of samples can be measured in clinical use. The system should contribute to an elucidation of the role of glycated lipoprotein in atherosclerosis.