Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative densitometric analysis using a commercially available handheld CCD digital camera

A M Malek1, S Izumo, S L Alper

  • 1Brigham & Women's Hospital/Children's Hospital, Department of Neurosurgery, Boston, MA 02115, USA.

Biotechniques
|June 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic Diseases of PIEZO1 and PIEZO2 Dysfunction.

Current topics in membranes·2017
Same author

The epidemiology of amyotrophic lateral sclerosis.

Handbook of clinical neurology·2016
Same author

BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins.

American journal of physiology. Cell physiology·2015
Same author

Clip occlusion versus coil embolization for the treatment of cerebral aneurysms.

Journal of neurosurgical sciences·2012
Same author

Assessment of heterologous membrane protein polarity in transiently transfected MDCK cells.

Cytotechnology·2012
Same author

Erratum Assessment of heterologous membrane protein polarity in transiently transfected MDCK cells.

Cytotechnology·2012

A new method uses a handheld digital camera for densitometric quantitation of radiographs. This digital imaging technique offers comparable results to traditional methods for laboratory applications.

Area of Science:

  • Biomedical imaging
  • Laboratory diagnostics
  • Radiographic analysis

Background:

  • Densitometric quantitation of radiographs is crucial in laboratory settings.
  • Traditional methods may be cumbersome or require specialized equipment.

Purpose of the Study:

  • To introduce and validate a novel method for radiographic densitometry using a handheld digital camera.
  • To assess the performance of this digital imaging technique against established methods.

Main Methods:

  • Acquisition of radiograph images using a handheld charge-coupled device (CCD) digital camera with fluorescent back-illumination.
  • Image analysis involving pixel intensity linearity checks and two-dimensional integration with background subtraction.
  • Comparison with a desktop scanner in transmittance mode and phosphor imaging.

Related Experiment Videos

Main Results:

  • Digital camera imaging demonstrated comparable linearity and quantitative accuracy to a desktop scanner.
  • The method showed similar performance to phosphor imaging over a limited dynamic range.
  • Pixel intensity analysis confirmed results within the linear response of radiographic film.

Conclusions:

  • Handheld digital imaging provides a viable and effective tool for quantitative densitometry in the laboratory.
  • This technology offers a potentially more accessible and adaptable alternative to existing densitometry techniques.
  • The study supports the integration of advanced digital imaging into standard laboratory workflows for radiographic analysis.