Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ARF1, a transcription factor that binds to auxin response elements

T Ulmasov1, G Hagen, T J Guilfoyle

  • 1Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, MO 65211, USA.

Science (New York, N.Y.)
|June 20, 1997
PubMed
Summary

Researchers identified Auxin Response Factor 1 (ARF1), a transcription factor crucial for plant hormone auxin regulation. ARF1 binds to specific DNA sequences, mediating gene expression in response to auxin signaling pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Magnetic Dipole Transition in ^{48}Ca.

Physical review letters·2024
Same author

Publisher Correction: First observation of <sup>28</sup>O.

Nature·2023
Same author

First observation of <sup>28</sup>O.

Nature·2023
Same author

β^{+} Gamow-Teller Strengths from Unstable ^{14}O via the (d,^{2}He) Reaction in Inverse Kinematics.

Physical review letters·2023
Same author

Trends of Neutron Skins and Radii of Mirror Nuclei from First Principles.

Physical review letters·2023
Same author

Proton Distribution Radii of ^{16-24}O: Signatures of New Shell Closures and Neutron Skin.

Physical review letters·2022

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Plant hormone auxin is vital for regulating physiological processes.
  • Auxin's action involves transcription factors interacting with auxin response elements (AuxREs) in genes.
  • Understanding these interactions is key to deciphering plant growth and development.

Purpose of the Study:

  • To clone and characterize a transcription factor involved in auxin response.
  • To investigate the DNA-binding properties and sequence specificity of the identified factor.
  • To explore the functional domains of the protein and their roles in auxin signaling.

Main Methods:

  • Cloning of Auxin Response Factor 1 (ARF1) from Arabidopsis using a yeast one-hybrid system.
  • In vitro analysis of ARF1's DNA-binding activity and sequence requirements.

Related Experiment Videos

  • Sequence homology analysis comparing ARF1 domains to known regulatory proteins.

    • Main Results:

    • ARF1 was successfully cloned and identified as a transcription factor binding to the TGTCTC sequence in AuxREs.
    • In vitro binding requirements for ARF1 precisely matched in vivo auxin responsiveness.
    • ARF1 possesses an N-terminal DNA-binding domain and a C-terminal domain with motifs common to Aux/IAA proteins, suggesting protein-protein interaction capabilities.

    Conclusions:

    • ARF1 is a key component in the auxin signaling pathway, directly linking auxin perception to gene expression.
    • The characterized DNA-binding specificity of ARF1 is critical for mediating auxin-regulated gene responses.
    • The protein-protein interaction domains in ARF1 suggest its integration into larger regulatory complexes within the auxin response machinery.