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Published on: December 20, 2013
Rhabdovirus-induced apoptosis in a fish cell line is inhibited by a human endogenous acid cysteine proteinase
H V Björklund1, T R Johansson, A Rinne
1Institute of Parasitology, Department of Biology, Abo Akademi University, Turku, Finland. harry.bjorklund@bmc.uu.se
Abstract:
To determine the mechanisms of cell death in rhabdovirus-infected cells, we studied the infection of the epithelial papilloma of carp cell line with spring viremia of carp virus. Studies using electron microscopy, confocal microscopy, and agarose gel electrophoresis revealed changes in cell morphology and DNA fragmentation indicative of apoptosis. The virus-induced apoptosis was inhibited in cells treated with a human endogenous acid cysteine proteinase inhibitor.
Insights
Spring viremia of carp virus induces apoptosis, a form of programmed cell death, in carp cells. This virus-induced apoptosis was blocked by a specific human proteinase inhibitor, revealing a potential therapeutic target.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Rhabdoviruses, such as spring viremia of carp virus (SVCV), can cause significant disease in fish.
- Understanding the host cell response, particularly cell death mechanisms, is crucial for managing viral infections.
Purpose of the Study:
- To investigate the mechanisms of cell death induced by SVCV in carp cells.
- To identify potential pathways involved in rhabdovirus-induced apoptosis.
Main Methods:
- Infection of epithelial papilloma of carp (EPC) cell line with SVCV.
- Utilized electron microscopy, confocal microscopy, and agarose gel electrophoresis to analyze cellular changes.
- Assessed the effect of a human endogenous acid cysteine proteinase inhibitor on virus-induced cell death.
Main Results:
- SVCV infection led to characteristic changes in cell morphology and DNA fragmentation, indicative of apoptosis.
- The observed virus-induced apoptosis was significantly inhibited by treatment with a human endogenous acid cysteine proteinase inhibitor.
Conclusions:
- Spring viremia of carp virus triggers apoptosis in infected carp cells.
- Acid cysteine proteinase inhibitors may play a role in regulating or preventing rhabdovirus-induced apoptosis, suggesting a potential therapeutic avenue.
