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Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase
Y Tian1, T W Beaman, S L Roderick
1Department of Biochemistry, Albert Einstein College of Medicine, The Bronx, New York 10461, USA.
Proteins
|June 1, 1997
Abstract:
A chloramphenicol acetyltransferase from Pseudomonas aeruginosa genomic DNA has been overexpressed, refolded, purified, and crystallized. Crystals suitable for a three-dimensional x-ray structure determination were obtained from solutions of polyethyleneglycol methyl ether 2000 containing NiCl2 at pH 8.5. These crystals belong to the cubic space group P4(1/3)32 (a = 154.8 A) and diffract x-rays to approximately 3.2 A resolution.