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Analytical partitioning of poly(ethylene glycol)-modified proteins
C Delgado1, M Malmsten, J M Van Alstine
1Molecular Cell Pathology, Royal Free Hospital School of Medicine, London, UK.
Abstract:
Covalently grafting proteins with varying numbers (n) of poly(ethylene glycol) molecules (PEGs) often enhances their biomedical and industrial usefulness. Partition between the phases in aqueous polymer two-phase systems can be used to rapidly characterize polymer-protein conjugates in a manner related to various enhancements. The logarithm of the partition coefficient (K) approximates linearity over the range O