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High-performance liquid chromatographic method for determination of DX-9065a, a novel anticoagulant, in human urine
1Daiichi Pharmaceutical Co. Ltd, Drug Metabolism and Analytical Chemistry Research Laboratory, Edogawa-ku, Tokyo, Japan.
Abstract:
A simple HPLC method for determination of DX-9065a in human urine and feces was developed. The drug was extracted by Bond Elut CBA, a cation-exchange solid-phase extraction cartridge. The extracted drug was analyzed by HPLC with UV detection at 242 nm. With this extraction procedure, no interfering peaks were observed. The method developed was validated and showed adequate precision and accuracy. This method was applied to human clinical samples obtained from healthy Japanese volunteers who had orally received the drug. Using this method, the excretion profile of the drug in human after oral administration was revealed for the first time.
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