Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleotide polymorphism in colicin E2 gene clusters: evidence for nonneutral evolution

Y Tan1, M A Riley

  • 1Department of Biology, Yale University, New Haven, Connecticut 06520-8104, USA.

Molecular Biology and Evolution
|June 1, 1997
PubMed
Summary

Colicin gene cluster evolution in Escherichia coli reveals two plasmid types. DNA sequence analysis indicates non-neutral evolution, driven by recombination and selection, not just random changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A case report of pulmonary sarcomatoid carcinoma with right tonsillar enlargement as the first diagnosis].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2025
Same author

Correction: CUL1 promotes trophoblast cell invasion at the maternal-fetal interface.

Cell death & disease·2025
Same author

Observation of the Very Rare Σ^{+}→pμ^{+}μ^{-} Decay.

Physical review letters·2025
Same author

Evidence for B^{-}→D^{**0}τ^{-}ν_{τ}[over ¯] Decays.

Physical review letters·2025
Same author

[Spousal correlations of blood lipid based on a family design].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2025
Same author

[Healthcare institution resilience and the influencing factors during infectious disease outbreaks].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2025

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Colicins are bacteriocins produced by Escherichia coli.
  • Colicin gene clusters exhibit significant diversity.
  • Understanding their evolution is key to microbial genetics.

Purpose of the Study:

  • To investigate the molecular mechanisms driving colicin gene cluster diversification.
  • To analyze DNA sequence polymorphism in colicin E2 (ColE2) plasmids.
  • To determine evolutionary forces acting on these gene clusters.

Main Methods:

  • Examined DNA sequence polymorphism of 14 ColE2 plasmids from natural E. coli isolates.
  • Compared polymorphism patterns between different ColE2 types and related colicin genes (e.g., ColE7, ColE9).

Related Experiment Videos

  • Applied statistical tests to assess evolutionary models (neutrality vs. non-neutrality).

    • Main Results:

    • Identified two distinct types of ColE2 plasmids, with type II arising from recombination between ColE2 and ColE7.
    • Observed differential DNA polymorphism patterns: even distribution in type I, concentrated at the 5' end in type II.
    • Found that divergence patterns do not correlate with intra-plasmid polymorphism, contradicting neutral evolution.

    Conclusions:

    • Recombination is a significant factor in the origin and diversification of type II ColE2 gene clusters.
    • Colicin gene clusters likely evolve under non-neutral evolutionary pressures, including selection.
    • These findings support the hypothesis of non-neutral forces shaping colicin gene cluster evolution.