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Expression of envelope protein (E2) of bovine viral diarrhea virus in insect cells
1National Veterinary Research Institute, Rural Development Administration, Anyang, Republic of Korea.
The Journal of Veterinary Medical Science
|May 1, 1997
Abstract:
The gene encoding the envelope glycoprotein (E2) of bovine viral diarrhea virus (BVDV) was expressed in a baculovirus. The expressed protein was detected on the surface of infected cells by immunofluorescence. Western blotting analysis showed the presence of the expressed protein of a similar molecular size to the E2 protein. The antigenicity of expressed protein were tested in guinea pigs and cattle. The immunized animals developed neutralizing antibodies against BVDV.