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Cloning of human chromosome 17-specific cDNAs using representational difference analysis and human-mouse hybrid cells
Y Tajima1, K Tashiro, D Camerini
1Department of Neurology, Hokkaido University School of Medicine, Sapporo, Japan.
Genomics
|June 1, 1997
Abstract:
We employed cDNA representational difference analysis (RDA) with human-mouse somatic hybrid cells containing human chromosome 17 and obtained several cDNA clones specific for this chromosome. A cDNA library from PHA-stimulated T cells was screened with unknown cDNA clones obtained by RDA as probes. Subsequently, 1 complete gene and 1 partial cDNA clone were obtained. Our successful result implies that this subtractive amplification technique with hybrid cells will be a useful aid in positional cloning in large-spanning regions.